No matter how effective the subject line you’ll always have subscribers who don’t open it for a variety of reasons. Send your email again specifically targeting a list segment of those who didn’t open the first time around. Not only is this a second chance in case they just missed the first email, it’s another opportunity to further split test subject lines as well as send times.
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Another disadvantage is that even an individual or small group of people can harm image of an established brand. For instance Dopplegnager is a term that is used to disapprove an image about a certain brand that is spread by anti-brand activists, bloggers, and opinion leaders. The word Doppelganger is a combination of two German words Doppel (double) and Ganger (walker), thus it means double walker or as in English it is said alter ego. Generally brand creates images for itself to emotionally appeal to their customers. However some would disagree with this image and make alterations to this image and present in funny or cynical way, hence distorting the brand image, hence creating a Doppelganger image, blog or content (Rindfleisch, 2016). http://www.thebridgecorp.com/wp-content/uploads/emailgraphic.png
To build a maling list, marketers collect information about clients and potential customers through in-house research. Many stores offer a substantial percentage off a first purchase when consumers apply for a credit card in the store, or when they opt-in to an email list. Other organizations and companies might set up a booth at a fair or conference, offering a chance to win an iPad when customers sign up for their newsletters and mailings.
InfusionSoft is a fully functional CRM and marketing solution created for small and medium size businesses. Comparing it to MailChimp or AWeber is like comparing a Lamborghini to a VW Golf. It provides highly advanced features for list segmentation, lead scoring and marketing automation. On top of all that, you can integrate all of these features with your optin form using Infusionsoft Tags + OptinMonster.

Particular groups of customers can be targeted or even individuals. Offering individual customers special deals on merchandise and/or services on the customer's birthday, for instance, is one example of email marketing personalization. (A restaurant might send an email to customers on their birthday offering 50% off an entree,) Email marketing helps a business develop and maintain a relationship with a customer over time that hopefully results in increased sales and increased customer loyalty. 
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